Preisanfragen ApexBio

Transcriptome sequencing is high-throughput sequencing of total RNA produced by a specific species or cell in a specific functional state, but now we mainly use it to detect mRNA.

APExBIO’s services not only detect differences in gene expression levels, but also provide structural analysis. Our work aims to help you discover unknown transcripts and rare transcripts, and accurately identify differentially variable splice sites, gene fusions, SNPs and InDel mutations, etc.  The results can be applied to research into growth and development regulation mechanisms and the pathogenesis of important human diseases.

 Metabolome and transcriptome sequence analysis reveals anthocyanin metabolism in pink flowers of anthocyanin-rich tea (Camellia sinensis)

Almost all flowers of the tea plant (Camellia sinensis) are white, which has led to few researchers paying attention to the accumulation of anthocyanins and color changes in tea flowers. A new purple leaf variety, Baitang Purple Tea (BTP), has been discovered in the Baitang Mountains of Guangdong, whose flowers are naturally pink, and may provide an opportunity to understand anthocyanin metabolic networks and the evolution of flower color in tea flowers. In the present study, twelve anthocyanin components were identified in the pink tea flowers, namely cyanidin-O-syringic acid, petunidin-3-O-glucoside, pelargonidin-3-O-beta-D-glucoside, which is the first time that these Compounds found were found in tea flowers. The presence of these anthocyanins seems most likely to be the reason for the pink coloring of the buds. Twenty-one differentially expressed genes (DEGs) involved in anthocyanin signaling were identified using functional enrichment of the KEGG signaling pathway,Specifically, during the period of peak anthocyanin synthesis, 17 structural genes were upregulated and only four structural genes were downregulated. Ultimately, eight critical genes were identified using weighted gene coexpression network analysis (WGCNA), which were found to have a direct impact on the biosynthesis and accumulation of three flavonoid compounds, namely cyanidin-3-O-glucoside, petunidin-3-O-glucoside and epicatechin gallate. These results provide useful information on the molecular mechanisms of coloration in rare pink tea flowers of anthocyanin-rich tea, enriching the gene resource and guiding further research on anthocyanin enrichment in violet tea.

The OPLS-DA results showed that the main biological components were significantly altered along with changing developmental stages.An OPLS-DA plot showing the significance of the change in metabolic profile from one developmental stage to the next. The composition between the first stage and the second stage (BTP1 vs. BTP2), the second stage and the third stage (BTP2 vs. BTP3), the third stage and the fourth stage (BTP3 vs. BTP4) and the fourth stage and the fifth stage (BTP4 vs. BTP5) each clustered together in the OPLS score plots. The R2Y of this OPLS-DA model was 1.0, 1.0, 0.999 and 1.0 in metabolomic differences of different flower development, respectively. While the model’s Q2Y was 0.992, 0.959, 0.938 and 0.975 (from BTP1 to BTP5) respectively. These data show highly significant differences in metabolite profiles based on developmental stage.

To reveal the gene expression levels involved in anthocyanins and flavonoid pathways, anthocyanin-associated modules were obtained from WGCNA. A module can be viewed as a cluster of closely related genes. The connectivity of two genes is a combination of the proximity between them and the strength of the connections they share with other “third party” genes. This measure of proximity used by WGCNA is known as the topological overlap measure (TOM). Using TOM, WGCNA groups data into dendrogram “tree” type. Individual branches of the tree represent clusters of interconnected genes, which are then defined as “modules”. Each module is measured for co-expression with the trait phenotype, in this case flavonoids, to see

The transcriptome response of cardiac and skeletal muscle to heat stress in low- and high-altitude-adapted Kenyan chicken ecotypes reveal differences in thermal tolerance and stress response

Heat stress (HS) has a negative impact on chicken performance. Agricultural expansion will take place in regions with high ambient temperatures where fast-growing commercial chickens are vulnerable. Indigenous chickens of such regions might have higher heat tolerance due to exposure to environmental issues over generations. In this study, two native chicken ecotypes,from the hot and humid Mombasa region (lowlands) and the colder Naivasha region (highlands) were used to assess the effects of acute (5 h, 35 °C) and chronic (3 days at 35 °C for 8 h/day) HS to be examined in heart and skeletal muscle by RNA sequencing. Rectal temperature rise and the number of differentially expressed genes (DEGs) [false discovery rate (FDR) < 0.05] were twice as high in the acute stage in both ecotypes as in the chronic stage, suggesting that cycling exposure to HS contributes may lead to adjustment. A tissue- and stage-specific difference in response to HS was observed, with peroxisome proliferator-activated receptor (PPAR) and mitogen-activated protein kinase (MAPK) signaling pathways in the heart and heart, respectively. Skeletal muscle and p53 enriched in both tissues only in the acute stage. The acute and chronic stage DEGs were integrated by a region-specific gene coexpression network (GCN), and genes with the highest number of connections (hub genes) were identified. The lowland network hub genes were CCNB2, Crb2, CHST9, SESN1, and NR4A3, while the highland network hub genes were COMMD4, TTC32, H1F0, ACYP1, and RPS28. Pathway analysis of genes in the GCN showed that p53 and PPAR signaling pathways were enriched in both Low and Highland networks, while MAPK signaling and protein processing in the endoplasmic reticulum were only enriched in the gene network of Highland chickens .

This shows that the ecotypes activated or repressed different genes, to dissipate accumulated heat, reduce heat-induced apoptosis, and promote DNA damage repair. indicating the differences in thermal tolerance and HS response mechanisms between the ecotypes. This study provides information on the HS response of chickens adapted to two different agroclimatic environments and advances our understanding of the mechanisms of the HS response and the impact of adaptation in controlling HS.

Rectal temperature rise during HS treatment and sample relationship derived from principal component analysis. (A) Box plot showing increase in rectal temperature due to HS treatment. AHL, acute highland; CHL, chronic highland; ALL, acute lowland; CLL, chronic lowland. (B) Principal component analysis showing that the maximum variation is due to differences between ecotypes. Only a small percentage of the variation is due to the HS effect.

PRICE DROP

110-012 GeneOn 4 x 1000 µl 159.6 EUR

PRICE DROP

110-012L GeneOn 5x 4 x 1000 µl 621.6 EUR

PRICE DROP

110-012XL GeneOn 10x 4 x 1000 µl 1126.8 EUR

ApexBlue™ Quick Cell Viability Fluorometric Assay Kit

K2023-2500 ApexBio 2500 assays 456 EUR

ApexBlue™ Quick Cell Viability Fluorometric Assay Kit

K2023-500 ApexBio 500 assays 206 EUR

ApexPrep DNA Plasmid Miniprep Kit

A5001-300 ApexBio 300 tests 160 EUR

ApexPrep DNA Plasmid Miniprep Kit

A5001-50 ApexBio 50 tests 32 EUR

ApexPrep DNA Plasmid Miniprep Column Only

A5002-300 ApexBio 300 tests 136 EUR

ApexPrep DNA Plasmid Miniprep Column Only

A5002-50 ApexBio 50 tests 26 EUR

Hafnium Pieces max. 55 mm appr. 100-300 g (price per weight)

GX6390-GX6390100g Glentham Life Sciences GX6390-100g 453 EUR

Hafnium Pieces max. 55 mm appr. 100-300 g (price per weight)

GX6390-GX6390250g Glentham Life Sciences GX6390-250g 857 EUR

Hafnium Pieces max. 55 mm appr. 100-300 g (price per weight)

GX6390-GX6390500g Glentham Life Sciences GX6390-500g 1505.5 EUR

JBScreen Plus Complete all 5 JBScreen Plus kits for a special price

M-CS-506 MiTeGen 5 Kits 1103 EUR

TARGATT ? Transgenic Kit (5 microinjections) Academic Price *Plasmid sold separately

AST-1003 Applied StemCell 1 Kit (5 microinjections) 691.8 EUR

TARGATT ? Transgenic Kit (2 microinjections) Academic Price *Plasmid sold separately

AST-1004 Applied StemCell 1 Kit (2 microinjections) 388.06 EUR

Serialized Engraving Service For Metal Grid Boxes - price is per grid box

M-CEM-AGG-Engrave MiTeGen 1 SERVICE 3 EUR

iPSC (iPSC from Blood Cells; Male, Master Lines for Neural Differentiation) *Academic Price

ASE-9109 Applied StemCell 1 vial (1 x 10^6) 1882.5 EUR

iPSC (iPSC from Blood Cells; Feale, Master Lines for Neural Differentiation) *Academic Price

ASE-9110 Applied StemCell 1 vial (1 x 10^6) 1882.5 EUR

APETx2

B5720-.1 ApexBio 100 ug 443 EUR

Apelin-13

C3633-1 ApexBio 1 mg 42 EUR

Apelin-13

C3633-10 ApexBio 10 mg 350 EUR

Apelin-13

C3633-25 ApexBio 25 mg 764 EUR

Apelin-13

C3633-5 ApexBio 5 mg 197 EUR

Custom Peptide Synthesis (crude and desalted; mg-kg size, price based upon peptide size: 2-100 aa)

PEP-C Alpha Diagnostics 1 Ask for price

[Pyr1]-Apelin-13

B7050-1 ApexBio 1 mg 133 EUR

[Pyr1]-Apelin-13

B7050-10 ApexBio 10mg 992 EUR

[Pyr1]-Apelin-13

B7050-5 ApexBio 5mg 530 EUR

Custom Peptide Synthesis (>90%; mass spec, hplc (mg-kg size, price based upon peptide size: 2-100 aa)

PEP-90 Alpha Diagnostics 1 Ask for price

Custom Peptide Synthesis (>95; mass spec, hplc (mg-kg size, price based upon peptide size: 2-100 aa)

PEP-95 Alpha Diagnostics 1 Ask for price

Apelin-36 (human)

B5303-.5 ApexBio 500 µg 331.2 EUR

Apelin-36 (human)

B5303-1 ApexBio 1 mg 554 EUR

Apelin-36 (human)

B5303-5 ApexBio 5 mg 2238 EUR

Apelin-36 (rat, mouse)

B5304-1 ApexBio 1 mg 602 EUR

Apelin-17 (human, bovine)

B5359-1 ApexBio 1 mg 330 EUR

Custom Peptide Conjugation to KLH/BSA/Ovalbumin or agarose (mg-100 mg size, price based upon servicesa)

CONJ-1 Alpha Diagnostics 1 Ask for price

PRIMA-1

A4483-10 ApexBio 10 mg 60 EUR

PRIMA-1

A4483-5 ApexBio 5 mg 45 EUR

PRIMA-1

A4483-5.1 ApexBio 10 mM (in 1mL DMSO) 40 EUR

PRIMA-1

A4483-50 ApexBio 50 mg 196 EUR

PRIMA-1

A4483-S ApexBio Evaluation Sample 22 EUR

PRIMA-1MET

A4484-10 ApexBio 10 mg 78 EUR

PRIMA-1MET

A4484-5.1 ApexBio 10 mM (in 1mL DMSO) 48 EUR

PRIMA-1MET

A4484-S ApexBio Evaluation Sample 22 EUR

Primidone

B2120-10 ApexBio 10 mg 153.6 EUR

Primidone

B2120-100 ApexBio 100mg 50 EUR

Primidone

B2120-5.1 ApexBio 10 mM (in 1mL DMSO) 40 EUR

Primidone

B2120-50 ApexBio 50 mg 362.4 EUR

Primidone

B2120-S ApexBio Evaluation Sample 22 EUR

Prilocaine

B2119-10 ApexBio 10 mg 32 EUR

Prilocaine

B2119-1000 ApexBio 1 g 40 EUR

Prilocaine

B2119-10000 ApexBio 10 g 192 EUR

Prilocaine

B2119-5.1 ApexBio 10 mM (in 1mL DMSO) 40 EUR

Prilocaine

B2119-5000 ApexBio 5 g 120 EUR

Pristimerin

B7488-10 ApexBio 10 mg 104 EUR

Pristimerin

B7488-25 ApexBio 25 mg 622.8 EUR

Pristimerin

B7488-5 ApexBio 5 mg 192 EUR

Pristimerin

B7488-50 ApexBio 50 mg 424 EUR

Prion Protein 106-126 (human)

B5433-.5 ApexBio 500 µg 301.2 EUR

Prion Protein 106-126 (human)

B5433-1 ApexBio 1 mg 302 EUR

Custom Peptide Synthesis (>70% antigen grade; mass spec, hplc (mg-kg size, price based upon peptide size: 2-100 aa)

PEP-70 Alpha Diagnostics 1 Ask for price

Primaquine Diphosphate

B1820-10000 ApexBio 10 g 128 EUR

Primaquine Diphosphate

B1820-5.1 ApexBio 10 mM (in 1mL DMSO) 48 EUR

Primaquine Diphosphate

B1820-5000 ApexBio 5 g 76 EUR

Prilocaine hydrochloride

B1280-10 ApexBio 10 mg 32 EUR

Prilocaine hydrochloride

B1280-1000 ApexBio 1 g 40 EUR

Prilocaine hydrochloride

B1280-10000 ApexBio 10 g 192 EUR

Prilocaine hydrochloride

B1280-5.1 ApexBio 10 mM (in 1mL DMSO) 40 EUR

Prilocaine hydrochloride

B1280-5000 ApexBio 5 g 120 EUR

Pridinol Methanesulfonate

A8507-1000 ApexBio 1 g 263 EUR

Pridinol Methanesulfonate

A8507-5.1 ApexBio 10 mM (in 1mL DMSO) 40 EUR

Pridinol Methanesulfonate

A8507-50 ApexBio 50 mg 54 EUR

Pridinol Methanesulfonate

A8507-5000 ApexBio 5 g 655 EUR

Prim-O-glucosylcimifugin

N1606-20 ApexBio 20 mg 120 EUR

Primary Antibody Dilution Buffer

K1200-100 ApexBio 100mL 192 EUR

Primary Antibody Dilution Buffer

K1200-500 ApexBio 500mL 672 EUR

Apex 500bp DNA Ladder

19-113 Genesee Scientific 1ml/Unit 132.31 EUR

Caspase-1, human recombinant proteinase

E1001-100 ApexBio 100 units 602.4 EUR

Caspase-1, human recombinant proteinase

E1001-25 ApexBio 25 units 245 EUR

Caspase-2, human recombinant proteinase

E1002-100 ApexBio 100 units 568.8 EUR

Caspase-2, human recombinant proteinase

E1002-25 ApexBio 25 units 322 EUR

Caspase-3, human recombinant proteinase

E1003-.005 ApexBio 5 µg 501.6 EUR

Caspase-3, human recombinant proteinase

E1003-.01 ApexBio 10 µg 802.8 EUR

Caspase-3, human recombinant proteinase

E1003-100 ApexBio 100 units 466 EUR

Caspase-3, human recombinant proteinase

E1003-25 ApexBio 25 units 250.8 EUR

Caspase-4, human recombinant protein

E1004-100 ApexBio 100 units 547 EUR

Caspase-4, human recombinant protein

E1004-25 ApexBio 25 units 351.6 EUR

Caspase-5, human recombinant protein

E1005-100 ApexBio 100 units 547 EUR

Caspase-5, human recombinant protein

E1005-25 ApexBio 25 units 351.6 EUR

Caspase-6, human recombinant protein

E1006-100 ApexBio 100 units 547 EUR

Caspase-6, human recombinant protein

E1006-25 ApexBio 25 units 351.6 EUR

Caspase-7, human recombinant protein

E1007-100 ApexBio 100 units 355 EUR

Caspase-7, human recombinant protein

E1007-25 ApexBio 25 units 206 EUR

Caspase-8, human recombinant protein

E1008-100 ApexBio 100 units 568.8 EUR

Caspase-8, human recombinant protein

E1008-25 ApexBio 25 units 322 EUR

Caspase-9, human recombinant protein

E1009-100 ApexBio 100 units 584.4 EUR

Caspase-9, human recombinant protein

E1009-25 ApexBio 25 units 384 EUR

Caspase-1, mouse recombinant protein

E1010-100 ApexBio 100 units 638 EUR

Caspase-1, mouse recombinant protein

E1010-25 ApexBio 25 units 351.6 EUR

Caspase-3 Proform, mouse recombinant protein

E1011-.005 ApexBio 5 µg 345 EUR

Caspase-10/a, human recombinant protein

E1012-100 ApexBio 100 units 542 EUR

Caspase-10/a, human recombinant protein

E1012-25 ApexBio 25 units 351.6 EUR

Caspase-10/b, human recombinant protein

E1013-100 ApexBio 100 units 542 EUR

Caspase-10/b, human recombinant protein

E1013-25 ApexBio 25 units 351.6 EUR

Phos binding reagent (Phosbind) Biotin

F4001-10 ApexBio 10 mg 400 EUR

Phos binding reagent (Phosbind) Biotin

F4001-5 ApexBio 5 mg 280 EUR

Phos binding reagent (Phosbind) Biotin

F4001-50 ApexBio 50 mg 1000 EUR

Phos binding reagent (Phosbind) acrylamide

F4002-10 ApexBio 10 mg 400 EUR

Phos binding reagent (Phosbind) acrylamide

F4002-5 ApexBio 5 mg 280 EUR

Phos binding reagent (Phosbind) acrylamide

F4002-50 ApexBio 50 mg 1000 EUR

Phos binding reagent (Phosbind) Biotin LC

F4004-10 ApexBio 10 mg 400 EUR

Phos binding reagent (Phosbind) Biotin LC

F4004-5 ApexBio 5 mg 280 EUR

Phos binding reagent (Phosbind) Biotin LC

F4004-50 ApexBio 50 mg 1000 EUR

Prestained Protein Marker (Triple color, EDTA free, 10-250 kDa)

F4005-.25 ApexBio 250ul 32 EUR

Prestained Protein Marker (Triple color, EDTA free, 10-250 kDa)

F4005-.5 ApexBio 2x250ul 58 EUR

Human ADM / Adrenomedullin Protein (Fc Tag)

G1001-.05 ApexBio 50 µg 787.2 EUR

Canine ANGPTL7 / Angiopoietin-like 7 Protein (Fc Tag)

G1002-.05 ApexBio 50 µg 787.2 EUR

Cynomolgus ANGPTL7 / Angiopoietin-like 7 Protein (His Tag)

G1003-.05 ApexBio 50 µg 787.2 EUR

Human Angiopoietin 4 / ANG4 / ANGPT4 Protein (His Tag)

G1004-.05 ApexBio 50 µg 560.4 EUR

Mouse Angiopoietin-2 / ANG2 / ANGPT2 Protein (His Tag)

G1005-.02 ApexBio 20 µg 710.4 EUR

Canine EGF / Epidermal Growth Facto Protein (His Tag)

G1006-.1 ApexBio 100 µg 153.6 EUR

Canine EGF / Epidermal Growth Facto Protein (His Tag)

G1006-.5 ApexBio 500 µg 309.6 EUR

Human EGF / Epidermal Growth Factor Protein (Fc Tag)

G1007-.5 ApexBio 500 µg 309.6 EUR

Mouse EGF / Epidermal Growth Factor Protein (Fc Tag)

G1008-.5 ApexBio 500 µg 285.6 EUR

Rat EGF / Epidermal Growth Factor Protein

G1009-.2 ApexBio 200 µg 560.4 EUR

Cynomolgus Erythropoietin / EPO Protein (His Tag)

G1010-.02 ApexBio 20 µg 787.2 EUR

Human EPOR / Erythropoietin Receptor Protein (Fc Tag)

G1011-.1 ApexBio 100 µg 835.2 EUR

Mouse Erythropoietin / EPO Protein

G1012-.02 ApexBio 20 µg 787.2 EUR

Rat Erythropoietin / EPO Protein (His Tag)

G1013-.02 ApexBio 20 µg 787.2 EUR

Human FGF21 Protein (His Tag)

G1014-.05 ApexBio 50 µg 536.4 EUR

Mouse FGF21 / Fibroblast Growth Factor 21 Protein (His Tag)

G1015-.05 ApexBio 50 µg 787.2 EUR

Canine HGF / Hepatocyte Growth Factor Protein

G1016-.05 ApexBio 50 µg 760.8 EUR

Cynomolgus HGF / Hepatocyte Growth Factor Protein

G1017-.02 ApexBio 20 µg 560.4 EUR

Human HGF / Hepatocyte Growth Factor Protein

G1018-.01 ApexBio 10 µg 331.2 EUR

Human HGF / Hepatocyte Growth Factor Protein

G1018-.05 ApexBio 50 µg 1179.6 EUR

Mouse GDF-8 / Myostatin / MSTN Protein (Fc Tag) (Cytokine)

G1021-.02 ApexBio 20 µg 861.6 EUR

Mouse SCF / C-kit ligand Protein (His Tag)

G1022-.005 ApexBio 5 µg 153.6 EUR

Mouse SCF / C-kit ligand Protein (His Tag)

G1022-.1 ApexBio 100 µg 1062 EUR

Adrenomedullin (1-12), human

A1001-10 ApexBio 10 mg 60 EUR

Adrenomedullin (1-12), human

A1001-25 ApexBio 25 mg 84 EUR

Adrenomedullin (1-12), human

A1001-5 ApexBio 5mg 36 EUR

Beta-Amyloid (1-11)

A1002-1 ApexBio 1 mg 36 EUR

Beta-Amyloid (1-11)

A1002-10 ApexBio 10 mg 180 EUR

Beta-Amyloid (1-11)

A1002-25 ApexBio 25 mg 252 EUR

Beta-Amyloid (1-11)

A1002-5 ApexBio 5 mg 108 EUR

Amyloid β-Protein (1-15)

A1003-1 ApexBio 1 mg 44 EUR

Amyloid β-Protein (1-15)

A1003-10 ApexBio 10 mg 220 EUR

Amyloid β-Protein (1-15)

A1003-25 ApexBio 25 mg 308 EUR

Amyloid β-Protein (1-15)

A1003-5 ApexBio 5 mg 132 EUR

Amyloid Precursor C-Terminal Peptide

A1004-1 ApexBio 1 mg 44 EUR

Amyloid Precursor C-Terminal Peptide

A1004-10 ApexBio 10 mg 220 EUR

Amyloid Precursor C-Terminal Peptide

A1004-25 ApexBio 25 mg 308 EUR

Amyloid Precursor C-Terminal Peptide

A1004-5 ApexBio 5 mg 132 EUR

Beta-Sheet Breaker Peptide iAβ5

A1005-10 ApexBio 10 mg 60 EUR

Beta-Sheet Breaker Peptide iAβ5

A1005-25 ApexBio 25 mg 84 EUR

Beta-Sheet Breaker Peptide iAβ5

A1005-5 ApexBio 5 mg 36 EUR

Angiotensin I (human, mouse, rat)

A1006-10 ApexBio 10 mg 80 EUR

Gene Coexpression Network (GCN) and pathway enrichment analysis integrated for the skeletal and cardiac muscle DEGs. (A) Degree sorted network of DEGs in at least one contrast in the highland chickens. The nodes are genes and the edges are based on correlation coefficients. Only partially correlated genes | r | of ≥0.99 were included in the network. The node color denotes the tissue type where gene expression was highest, while the node border denotes the stage where gene expression was highest.

(B) KEGG pathway networks in which all genes in the upland GCN network have been enriched. (c) Degree sorted network of the DEG in at least one contrast in the lowland chickens. The nodes are genes and the edges are based on correlation coefficients. Only partially correlated genes | r | from ≥0, 99 were added to the network. The node color denotes the tissue type where gene expression was highest, while the node border denotes the stage where gene expression was highest. (D) KEGG pathway networks in which all genes in the lowland GCN network have been enriched.

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