Hevert-Arzneimittel Blog Detectx testosterone Elisa kit

Detectx testosterone Elisa kit

The DetectX Testosterone Immunoassay kit uses a specifically generated antibody to measure testosterone and its metabolites in urine and fecal samples, or in extracted serum and plasma. This kit is not recommended for serum, plasma, or saliva samples without extraction.

The kit will quantitatively measure Testosterone present in reconstituted buffer samples and tissue culture media samples.

A testosterone standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A testosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to testosterone to each well. After a 2 hour incubation, the plate is washed and the substrate is added.

The substrate reacts with the bound testosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength.

The DetectX Testosterone ELISA Kit uses an antibody specifically generated to measure testosterone and its metabolites in urine and stool samples, or in extracted serum and plasma. This kit is not recommended for serum, plasma, or saliva samples without extraction.

The kit will quantitatively measure the testosterone present ELISA Kit in reconstituted buffer samples and tissue culture media samples. A testosterone standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture the rabbit antibodies. A testosterone-peroxidase conjugate is added to the standards and samples in the wells.

The binding reaction is initiated by adding a polyclonal antibody to testosterone in each well. After 2 hours of incubation, the plate is washed and the substrate is added. The substrate reacts with the bound testosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the color generated is detected on a microtiter plate reader capable of measuring a wavelength of 450 nm.

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