If you are working with DNA, you may be looking for an easier and more efficient way to extract it. With increased accessibility to computer-controlled instruments and reagents, as well as development of specialized protocols, there have been many advances in the field of biotechnology in recent years. One such advancement is the Viagen DirectPCR DNA Extraction System, which allows you to quickly prepare high quality DNA from various samples types including mouse tails, ear pieces, yolk sacs, and culture cells.
Why Use Viagen DirectPCR DNA Extraction System?
There are many reasons to use the Viagen DirectPCR DNA Extraction System. This system is PCR ready, which means that it is able to be used with a polymerase chain reaction machine. This system is also able to extract DNA from a variety of different sources, including blood, tissue, and even plant material. In addition, this system is very easy to use and can be completed in just a few minutes. Who Needs Viagen DirectPCR DNA Extraction System?. Viagen DirectPCR DNA Extraction System is recommended for anyone who needs to extract DNA samples from a sample. Whether you are a researcher, scientist, forensic expert, or even simply someone looking to learn more about genetics and genealogy, this system is useful for everyone.
What Are the Features of Viagen DirectPCR DNA Extraction System?. Here are some of the key features of this system:
• PCR ready – includes all necessary components to use with polymerase chain reaction machines
• Can extract DNA from blood and tissue samples as well as plant material such as seeds or leaves
• Extracts less than one microgram of DNA
How to Use Viagen DirectPCR DNA Extraction System
If you’re looking for a quick and easy way to extract DNA from cells, the Viagen DirectPCR system is perfect for you. This PCR-ready system can be used with any cell type and doesn’t require any special equipment or knowledge – all you need is a few minutes and some basic lab supplies. Here’s how it works:
1) First, add the appropriate amount of lysis buffer to your sample. Lysis buffer breaks open cells and releases their contents, including DNA.
2) Next, incubate the sample at 95°C for 10 minutes. This step denatures proteins that could interfere with DNA extraction.
3) Then, add the DirectPCR enzyme mix to the sample and incubate for 5 minutes at room temperature. The enzymes in the mix will bind to and break down DNA.
4) Finally, add your favorite PCR master mix and run your PCR reaction as usual. You’ll now have PCR-ready DNA that’s ready to use in any experiment! DirectPCR® is an enzyme mix that allows you to directly purify DNA from cells, tissues, or plant material in just minutes. The kit also includes a special buffer that eliminates the need for extraction and precipitation steps during PCR setup. DirectPCR uses a proprietary blend of enzymes to break down DNA into smaller fragments that are compatible with PCR assays. Use this handy kit to generate your own genomic DNA for applications like genotyping and sequencing!
Note: This product is supplied as a dry powder. It will be necessary to add water before use.
Where to Buy the Viagen DirectPCR DNA Extraction System?
If you’re looking for a PCR-ready DNA extraction system, the Viagen DirectPCR system is a great option. This system is designed for use with whole blood, so it’s perfect for extracting DNA from samples that contain blood cells. The Viagen DirectPCR system is available from several different vendors, so you can choose the one that best suits your needs. For example:
Viagen DirectPCR DNA Extraction System (Cat # V5202) is available from B&J Biotech for $1,400.00. This kit includes a 96-well Thermal Cycler with a software package that allows you to monitor PCR reactions remotely via the web. In addition, it includes all the supplies needed to prepare and purify genomic DNA from whole blood samples. The Viagen DirectPCR system has been tested for use with human blood samples, but you will need to purchase the BioRobot eXtractor machine separately if you want to process samples of other species. This system comes with everything you need to start extracting DNA from whole blood samples in order to do PCR testing.
DIRECTPCR LYSIS REAGENT (TAIL) | ||||
101-T | Cusabio | 50 ml | 169.2 EUR | |
DIRECTPCR LYSIS REAGENT (TAIL) | ||||
102-T | Viagen | 100ml | 248.4 EUR | |
DIRECTPCR LYSIS REAGENT (EAR) | ||||
401-E | Viagen | 25 ml | 169.2 EUR | |
DIRECTPCR LYSIS REAGENT (EAR) | ||||
402-E | Viagen | 50 ml | 248.4 EUR | |
DIRECTPCR LYSIS REAGENT (CELL) | ||||
301-C | Viagen | 50 ml | 169.2 EUR | |
DIRECTPCR LYSIS REAGENT (CELL) | ||||
302-C | Viagen | 100 ml | 248.4 EUR | |
DetectX® Creatinine Reagent, 50ML | ||||
C004-50ML | Arbor Assays | 50ML | 430.8 EUR | |
DIRECTPCR LYSIS RGNT(YOLK SAC) | ||||
201-Y | Viagen | 50 ml | 169.2 EUR | |
DIRECTPCR LYSIS YOLK SAC 100ML | ||||
202-Y | Viagen | 100 ml | 248.4 EUR | |
Reagent Reservoir 50ml | ||||
F267670 | Scientific Laboratory Supplies | PK100 | 232.56 EUR | |
RBC Lysis Solution 10X | ||||
abx290033-50ml | Abbexa | 50 ml | 276 EUR | |
RBC Lysis Solution 10X | ||||
RBC10X-50ML | ImmunoStep | 50 ML | 130.2 EUR | |
mRNAExpress Tail PCR mix (5uM) | ||||
MR-TAIL-PR | SBI | 100 ul | 196.8 EUR | |
Assay Buffer, 50ML | ||||
X100-50ML | Arbor Assays | 50ML | 130.8 EUR | |
Assay Buffer, 50ML | ||||
X117-50ML | Arbor Assays | 50ML | 171.6 EUR | |
Extraction Solution, 50ML | ||||
X123-50ML | Arbor Assays | 50ML | 147.6 EUR | |
Purified Water, 50ML | ||||
X137-50ML | Arbor Assays | 50ML | 146.4 EUR | |
TARGATT? H11 Mouse Tail Lysis PCR Kit | ||||
AST-2005 | Applied StemCell | 100 reactions | 156.72 EUR | |
TARGATT? Rosa26 Mouse Tail Lysis PCR Kit | ||||
AST-2006 | Applied StemCell | 100 reactions | 156.72 EUR | |
Hemoglobin Sample Diluent, 50ML | ||||
X052-50ML | Arbor Assays | 50ML | 183.6 EUR | |
Bottle Pyrex Reagent 50ml. | ||||
1516/02D | Scientific Laboratory Supplies | PK10 | 92.76 EUR | |
Bottle Reagent Simax 50ml | ||||
BOT53020 | Scientific Laboratory Supplies | PK10 | 56.66 EUR | |
Allele-In-One Mouse Tail Direct Lysis Buffer | ||||
ABP-PP-MT01100 | Allele Biotech | 100 RXNS | Ask for price | |
Allele-In-One Mouse Tail Direct Lysis Buffer | ||||
ABP-PP-MT01500 | Allele Biotech | 500 RXNS | Ask for price | |
Folin & Ciocalteu’s phenol reagent | ||||
abx082175-50ml | Abbexa | 50 ml | 226.8 EUR | |
SingleStep Poly-HRP Anti-Mouse IgG (H+L) Secondary Antibody, HRP-50ml | ||||
Q2AB1-50ml | EnQuireBio | 50ml | 483.6 EUR | |
Xpro IP Cell Lysis Reagent,SDS Free | ||||
X3100-010 | GenDepot | 100ml | 208.8 EUR | |
Xpro IP Cell Lysis Reagent,SDS Free | ||||
X3100-050 | GenDepot | 500ml | 380.4 EUR | |
50ML , CONICAL BOTTOMED TUBE WITH CAP, UNASSEMBLED | ||||
SCT-50ML-500 | CORNING | 50/pk | 104.4 EUR | |
SingleStep Poly-HRP Anti-Rabbit IgG (H+L) Secondary Antibody, HRP-50ml | ||||
Q2AB2-50ml | EnQuireBio | 50ml | 483.6 EUR | |
Costar 50mL Reagent Reservoirs 5/Bag Sterile | ||||
4870 | Scientific Laboratory Supplies | PK200 | 279.3 EUR | |
Costar 50mL Reagent Reservoirs 1/Bag Sterile | ||||
4871 | Scientific Laboratory Supplies | PK100 | 323.76 EUR | |
Simax Reagent Bottle Narrow Mouth Amber 50ml | ||||
BOT64222 | Scientific Laboratory Supplies | PK10 | 52.1 EUR | |
Select Reagent Bottle Wide Mouth Clear 50ml | ||||
BOT64420 | Scientific Laboratory Supplies | PK10 | 52.1 EUR | |
Reagent Bottle Clear Narrow neck with plastic stopper 50ml | ||||
BOT2140 | Scientific Laboratory Supplies | PK10 | 38.19 EUR | |
Reagent Bottle Amber Narrow neck with plastic stopper 50ml | ||||
BOT2156 | Scientific Laboratory Supplies | PK10 | 44.57 EUR | |
50ML , CONICAL BOTTOMED TUBE WITH CAP, ASSEMBLED, PRE-STERILIZED | ||||
SCT-50ML-25-S | CORNING | 25/pk | 117.6 EUR | |
50ML , CONICAL BOTTOMED TUBE WITH CAP, RACKED, ASSEMBLED, PRE-STERILIZED | ||||
SCT-50ML-R-S | CORNING | 250/pk | 127.2 EUR | |
Lysis Buffer | ||||
abx098984-LysisBuffer120ml | Abbexa | Lysis Buffer 1 (20 ml) | 184.8 EUR | |
Lysis Buffer | ||||
abx098984-LysisBuffer3100ml | Abbexa | Lysis Buffer 3 (100ml) | 276 EUR | |
Lysis Buffer | ||||
abx098984-LysisBuffer420ml | Abbexa | Lysis Buffer 4 (20 ml) | 184.8 EUR | |
EP Reagent Biuret Reagent | ||||
1011601 | Scientific Laboratory Supplies | 1L | 42.18 EUR | |
EP Reagent Iodoplatinate Reagent | ||||
1046300 | Scientific Laboratory Supplies | 200ML | 360.24 EUR | |
EP Reagent Methoxyphenylacetic Reagent | ||||
1053601 | Scientific Laboratory Supplies | 100ML | 354.54 EUR | |
EP Reagent Molybdovanadic Reagent | ||||
1056700 | Scientific Laboratory Supplies | 100ML | 42.18 EUR | |
EP Reagent Phosphomolybdotungstic Reagent | ||||
1065000 | Scientific Laboratory Supplies | 100ML | 161.88 EUR | |
CometAssay Lysis Solution | ||||
4250-010-01 | Biotechne | 100 mL | 189.36 EUR | |
Cell Lysis Buffer | ||||
AR0103 | BosterBio | 100mL (The kit contains sufficient lysis buffer for 200 cell pellet fractions containing 5x 106 cells each) | 145.2 EUR | |
RIPA Lysis Buffer | ||||
AR0105 | BosterBio | 50 mL | 90 EUR | |
IP Lysis Buffer | ||||
AR0107 | BosterBio | 100mL | 121.2 EUR | |
ExCellenCT Lysis Kit | ||||
G915 | ABM | 25 Preps | 110.4 EUR | |
Cell Lysis Buffer | ||||
GR103003 | Genorise Scientific | 200 mL | 64.8 EUR | |
Cell Lysis Buffer | ||||
1067-100 | Biovision | each | 196.8 EUR | |
Cell Lysis Buffer | ||||
1067-400 | Biovision | each | 352.8 EUR | |
Mouse Collagen I (Tail Tendon) Protein | ||||
abx670020-05mg | Abbexa | 0.5 mg | 427.2 EUR | |
Mouse IgG Blocking Reagent | ||||
30R-AH071 | Fitzgerald | 20 mg | 309.6 EUR | |
EP Reagent Sulfomolybdic Reagent R3 | ||||
1086500 | Scientific Laboratory Supplies | 1L | 287.28 EUR | |
Mouse-to-Mouse Blocking Reagent | ||||
MTM008 | ScyTek Laboratories | 8 ml | 96 EUR | |
Mouse-to-Mouse Blocking Reagent | ||||
MTM015 | ScyTek Laboratories | 15 ml | 105.6 EUR | |
Mouse-to-Mouse Blocking Reagent | ||||
MTM125 | ScyTek Laboratories | 125 ml | 434.4 EUR | |
Mouse-to-Mouse Blocking Reagent | ||||
MTM500 | ScyTek Laboratories | 500 ml | 991.2 EUR | |
CD2 Tail-binding antibody | ||||
70R-32334 | Fitzgerald | 100 ug | 392.4 EUR | |
CD2 Tail-binding Antibody | ||||
45412-100ul | SAB | 100ul | 302.4 EUR | |
CD2 Tail-binding Antibody | ||||
45412-50ul | SAB | 50ul | 224.4 EUR | |
CD2 Tail-binding Antibody | ||||
34187-100ul | SAB | 100ul | 302.4 EUR | |
CD2 Tail-binding Antibody | ||||
34187-50ul | SAB | 50ul | 224.4 EUR | |
CD2 Tail- binding Antibody | ||||
ABD8644 | Lifescience Market | 100 ug | 525.6 EUR | |
CD2 Tail- binding Antibody | ||||
ABD13035 | Lifescience Market | 100 ug | 525.6 EUR | |
CD2 Tail-binding Antibody | ||||
DF8644 | Affbiotech | 200ul | 420 EUR | |
Probe Fish Tail 150mm | ||||
INS6110 | Scientific Laboratory Supplies | EACH | 16.2 EUR | |
Probe Fish Tail 200mm | ||||
INS6112 | Scientific Laboratory Supplies | EACH | 16.2 EUR | |
Probe Fish Tail 250mm | ||||
INS6114 | Scientific Laboratory Supplies | EACH | 16.14 EUR | |
RIPA Lysis Buffer (Strong) | ||||
abx090624-100ml | Abbexa | 100 ml | 260.4 EUR | |
RIPA Lysis Buffer (Medium) | ||||
abx090625-100ml | Abbexa | 100 ml | 260.4 EUR | |
RIPA Lysis Buffer (Weak) | ||||
abx090626-100ml | Abbexa | 100 ml | 260.4 EUR | |
5X Passive Lysis Buffer | ||||
99912 | Biotium | 30ML | 92.4 EUR | |
CHAPS Cell Lysis Buffer | ||||
C2000-100 | GenDepot | 100ml | 165.6 EUR | |
CHAPS Cell Lysis Buffer | ||||
C2000-500 | GenDepot | 500ml | 400.8 EUR | |
RoomTemp Sample Lysis Kit | ||||
P073-01 | Vazyme | 250 rxn (5 ml) | 254.4 EUR | |
RoomTemp Sample Lysis Kit | ||||
P073-02 | Vazyme | 1000 rxn ( 20 ml) | 567.6 EUR | |
RoomTemp Sample Lysis Kit | ||||
P073-03 | Vazyme | 5000 rxn (100 ml) | 1680 EUR | |
Orange Lysis Kit 50 | ||||
ORANGEE1-RNA | Next Advance | 50pack | 198 EUR | |
Orange Lysis Kit 250 | ||||
ORANGEE5-RNA | Next Advance | 250pack | 644.4 EUR | |
Navy Lysis Kit 50 | ||||
NAVYE1 | Next Advance | 50pack | 210 EUR | |
Navy Lysis Kit 250 | ||||
NAVYE5 | Next Advance | 250pack | 656.4 EUR | |
1X Protein Lysis Buffer | ||||
G032 | ABM | 5.0 ml (5 x 1.0ml) | 92.4 EUR | |
RIPA Lysis Buffer (Strong) | ||||
HY-K1001 | MedChemExpress | 100 mL | 176.4 EUR | |
Green Lysis Kit 50 | ||||
GREENE1 | Next Advance | 50pack | 187.2 EUR | |
Green Lysis Kit 250 | ||||
GREENE5 | Next Advance | 250pack | 561.6 EUR | |
Pink Lysis Kit 50 | ||||
PINKE1 | Next Advance | 50pack | 184.8 EUR | |
Pink Lysis Kit 250 | ||||
PINKE5 | Next Advance | 250pack | 548.4 EUR | |
Red Lysis Kit 50 | ||||
REDE1 | Next Advance | 50pack | 201.6 EUR | |
Red Lysis Kit 250 | ||||
REDE5 | Next Advance | 250pack | 624 EUR | |
Cell Lysis Buffer, 100ML | ||||
X050-100ML | Arbor Assays | 100ML | 130.8 EUR | |
YELLOW Lysis Kit 50 | ||||
YELLOWE1 | Next Advance | 50pack | 210 EUR | |
YELLOW Lysis Kit 250 | ||||
YELLOWE5 | Next Advance | 250pack | 657.6 EUR | |
Mouse Sickle tail protein (SKT) ELISA Kit | ||||
abx389166-96tests | Abbexa | 96 tests | 1093.2 EUR | |
Mouse Tail Genomic DNA Extraction Kit(250) | ||||
M9100-250 | GenDepot | 250 prep | 643.2 EUR | |
Mouse Sickle tail protein, Skt ELISA KIT | ||||
ELI-29595m | Lifescience Market | 96 Tests | 1038 EUR | |
BOP reagent | ||||
5-02141 | CHI Scientific | 25g | Ask for price |
Comparison of Single-Tube Systems
The Viagen DirectPCR system is a single-tube DNA extraction system that is PCR-ready. This system is compared to other single-tube systems on the market, and it is found to be more efficient and easier to use. The Viagen DirectPCR system can be used with a variety of sample types, and it provides high yields of DNA. This system is also relatively inexpensive, making it a good option for those who are looking for an affordable DNA extraction system.