If you are working with DNA, you may be looking for an easier and more efficient way to extract it. With increased accessibility to computer-controlled instruments and reagents, as well as development of specialized protocols, there have been many advances in the field of biotechnology in recent years. One such advancement is the Viagen DirectPCR DNA Extraction System, which allows you to quickly prepare high quality DNA from various samples types including mouse tails, ear pieces, yolk sacs, and culture cells.
Why Use Viagen DirectPCR DNA Extraction System?
There are many reasons to use the Viagen DirectPCR DNA Extraction System. This system is PCR ready, which means that it is able to be used with a polymerase chain reaction machine. This system is also able to extract DNA from a variety of different sources, including blood, tissue, and even plant material. In addition, this system is very easy to use and can be completed in just a few minutes. Who Needs Viagen DirectPCR DNA Extraction System?. Viagen DirectPCR DNA Extraction System is recommended for anyone who needs to extract DNA samples from a sample. Whether you are a researcher, scientist, forensic expert, or even simply someone looking to learn more about genetics and genealogy, this system is useful for everyone.
What Are the Features of Viagen DirectPCR DNA Extraction System?. Here are some of the key features of this system:
• PCR ready – includes all necessary components to use with polymerase chain reaction machines
• Can extract DNA from blood and tissue samples as well as plant material such as seeds or leaves
• Extracts less than one microgram of DNA
How to Use Viagen DirectPCR DNA Extraction System
If you’re looking for a quick and easy way to extract DNA from cells, the Viagen DirectPCR system is perfect for you. This PCR-ready system can be used with any cell type and doesn’t require any special equipment or knowledge – all you need is a few minutes and some basic lab supplies. Here’s how it works:
1) First, add the appropriate amount of lysis buffer to your sample. Lysis buffer breaks open cells and releases their contents, including DNA.
2) Next, incubate the sample at 95°C for 10 minutes. This step denatures proteins that could interfere with DNA extraction.
3) Then, add the DirectPCR enzyme mix to the sample and incubate for 5 minutes at room temperature. The enzymes in the mix will bind to and break down DNA.
4) Finally, add your favorite PCR master mix and run your PCR reaction as usual. You’ll now have PCR-ready DNA that’s ready to use in any experiment! DirectPCR® is an enzyme mix that allows you to directly purify DNA from cells, tissues, or plant material in just minutes. The kit also includes a special buffer that eliminates the need for extraction and precipitation steps during PCR setup. DirectPCR uses a proprietary blend of enzymes to break down DNA into smaller fragments that are compatible with PCR assays. Use this handy kit to generate your own genomic DNA for applications like genotyping and sequencing!
Note: This product is supplied as a dry powder. It will be necessary to add water before use.
Where to Buy the Viagen DirectPCR DNA Extraction System?
If you’re looking for a PCR-ready DNA extraction system, the Viagen DirectPCR system is a great option. This system is designed for use with whole blood, so it’s perfect for extracting DNA from samples that contain blood cells. The Viagen DirectPCR system is available from several different vendors, so you can choose the one that best suits your needs. For example:
Viagen DirectPCR DNA Extraction System (Cat # V5202) is available from B&J Biotech for $1,400.00. This kit includes a 96-well Thermal Cycler with a software package that allows you to monitor PCR reactions remotely via the web. In addition, it includes all the supplies needed to prepare and purify genomic DNA from whole blood samples. The Viagen DirectPCR system has been tested for use with human blood samples, but you will need to purchase the BioRobot eXtractor machine separately if you want to process samples of other species. This system comes with everything you need to start extracting DNA from whole blood samples in order to do PCR testing.
DIRECTPCR LYSIS REAGENT (TAIL) | ||||
| 101-T | Cusabio | 50 ml | 169.2 EUR | |
DIRECTPCR LYSIS REAGENT (TAIL) | ||||
| 102-T | Viagen | 100ml | 248.4 EUR | |
DIRECTPCR LYSIS REAGENT (EAR) | ||||
| 401-E | Viagen | 25 ml | 169.2 EUR | |
DIRECTPCR LYSIS REAGENT (EAR) | ||||
| 402-E | Viagen | 50 ml | 248.4 EUR | |
DIRECTPCR LYSIS REAGENT (CELL) | ||||
| 301-C | Viagen | 50 ml | 169.2 EUR | |
DIRECTPCR LYSIS REAGENT (CELL) | ||||
| 302-C | Viagen | 100 ml | 248.4 EUR | |
DIRECTPCR LYSIS YOLK SAC 100ML | ||||
| 202-Y | Viagen | 100 ml | 248.4 EUR | |
DIRECTPCR LYSIS RGNT(YOLK SAC) | ||||
| 201-Y | Viagen | 50 ml | 169.2 EUR | |
Allele-In-One Mouse Tail Direct Lysis Buffer | ||||
| ABP-PP-MT01100 | Allele Biotech | 100 RXNS | Ask for price | |
Allele-In-One Mouse Tail Direct Lysis Buffer | ||||
| ABP-PP-MT01500 | Allele Biotech | 500 RXNS | Ask for price | |
Xpro IP Cell Lysis Reagent,SDS Free | ||||
| X3100-010 | GenDepot | 100ml | 208.8 EUR | |
Xpro IP Cell Lysis Reagent,SDS Free | ||||
| X3100-050 | GenDepot | 500ml | 380.4 EUR | |
Reagent Reservoir 50ml | ||||
| F267670 | Scientific Laboratory Supplies | PK100 | 262.8 EUR | |
Allele-In-One Mouse Tail Direct PCR Kit | ||||
| ABP-PP-MT02100 | Allele Biotech | 100 RXNS | Ask for price | |
Allele-In-One Mouse Tail Direct PCR Kit | ||||
| ABP-PP-MT02500 | Allele Biotech | 500 RXNS | Ask for price | |
Bottle Pyrex Reagent 50ml. | ||||
| 1516/02D | Scientific Laboratory Supplies | PK10 | 106.8 EUR | |
Bottle Reagent Simax 50ml | ||||
| BOT53020 | Scientific Laboratory Supplies | PK10 | 66 EUR | |
amfiSure Direct PCR Lysis Buffer | ||||
| D0300-010 | GenDepot | 100ml | 165.6 EUR | |
Tail Lysis Buffer | ||||
| 06169-95 | NACALAI TESQUE | 500ML | 61.6 EUR | |
DetectX® Creatinine Reagent, 50ML | ||||
| C004-50ML | Arbor Assays | 50ML | 360 EUR | |
TARGATT? H11 Mouse Tail Lysis PCR Kit | ||||
| AST-2005 | Applied StemCell | 100 reactions | 156.72 EUR | |
TARGATT? Rosa26 Mouse Tail Lysis PCR Kit | ||||
| AST-2006 | Applied StemCell | 100 reactions | 156.72 EUR | |
Tail Lysis Buffer 1 | ||||
| 10450042-1 | Glycomatrix | 250 mL | 36.05 EUR | |
Tail Lysis Buffer 1 | ||||
| 10450042-2 | Glycomatrix | 500 mL | 54.54 EUR | |
Tail Lysis Buffer 2 | ||||
| 10450044-1 | Glycomatrix | 250 ml | 27.17 EUR | |
Tail Lysis Buffer 2 | ||||
| 10450044-2 | Glycomatrix | 500 ml | 45.38 EUR | |
Tail Lysis Buffer 3 | ||||
| 10450046-1 | Glycomatrix | 250 ml | 27.17 EUR | |
Tail Lysis Buffer 3 | ||||
| 10450046-2 | Glycomatrix | 500 ml | 54.54 EUR | |
Tail Lysis Buffer 4 | ||||
| 10450048-1 | Glycomatrix | 500 ml | 45.48 EUR | |
Select Reagent Bottle Wide Mouth Clear 50ml | ||||
| BOT64420 | Scientific Laboratory Supplies | PK10 | 61.2 EUR | |
Simax Reagent Bottle Narrow Mouth Amber 50ml | ||||
| BOT64222 | Scientific Laboratory Supplies | PK10 | 61.2 EUR | |
Tail Lysis Buffer 1 2X | ||||
| 10450043-1 | Glycomatrix | 250 ml | 54.54 EUR | |
Tail Lysis Buffer 1 2X | ||||
| 10450043-2 | Glycomatrix | 500 ml | 89.32 EUR | |
Tail Lysis Buffer 2 2X | ||||
| 10450045-1 | Glycomatrix | 250 ml | 27.17 EUR | |
Tail Lysis Buffer 2 2X | ||||
| 10450045-2 | Glycomatrix | 500 ml | 45.38 EUR | |
Tail Lysis Buffer 3 2X | ||||
| 10450047-1 | Glycomatrix | 500 ml | 54.54 EUR | |
Tail Lysis Buffer 4 2X | ||||
| 10450049-1 | Glycomatrix | 500 ml | 63.61 EUR | |
Costar 50mL Reagent Reservoirs 5/Bag Sterile | ||||
| 4870 | Scientific Laboratory Supplies | PK200 | 298.8 EUR | |
Costar 50mL Reagent Reservoirs 1/Bag Sterile | ||||
| 4871 | Scientific Laboratory Supplies | PK100 | 346.8 EUR | |
Alkphos Direct Labelling Reagents | ||||
| RPN3680 | Scientific Laboratory Supplies | EACH | 813.6 EUR | |
Reagent Bottle Clear Narrow neck with plastic stopper 50ml | ||||
| BOT2140 | Scientific Laboratory Supplies | PK10 | 40.2 EUR | |
Reagent Bottle Amber Narrow neck with plastic stopper 50ml | ||||
| BOT2156 | Scientific Laboratory Supplies | PK10 | 46.92 EUR | |
BCA Reagent, 16ML | ||||
| C144-16ML | Arbor Assays | 16ML | 132 EUR | |
TDA Reagent - 10mL | ||||
| MIC6732 | Scientific Laboratory Supplies | EACH | 26.33 EUR | |
PYR Reagent - 10mL | ||||
| MIC6744 | Scientific Laboratory Supplies | EACH | 31.12 EUR | |
Bottle Reagent 500ml Pyrex | ||||
| B54564 | Scientific Laboratory Supplies | PK10 | 124.8 EUR | |
MIDASplus 10mL Single Reagent 100mL | ||||
| M-MDSR-106 | MiTeGen | 100 ml ml | 183 EUR | |
Color Reagent A, 15ML | ||||
| X094-15ML | Arbor Assays | 15ML | 59 EUR | |
Color Reagent A, 38ML | ||||
| X094-38ML | Arbor Assays | 38ML | 120 EUR | |
Color Reagent B, 15ML | ||||
| X095-15ML | Arbor Assays | 15ML | 59 EUR | |
Color Reagent B, 38ML | ||||
| X095-38ML | Arbor Assays | 38ML | 120 EUR | |
Bottle Reagent Pyrex 100ml. | ||||
| B54558 | Scientific Laboratory Supplies | PK10 | 90 EUR | |
Simax Reagent Bottle 100ml | ||||
| BOT53040 | Scientific Laboratory Supplies | PK10 | 28.8 EUR | |
Simax Reagent Bottle 250ml | ||||
| BOT53060 | Scientific Laboratory Supplies | PK10 | 33.6 EUR | |
Simax Reagent Bottle 500ml | ||||
| BOT5308 | Scientific Laboratory Supplies | EACH | 4.19 EUR | |
Simax Reagent Bottle 500ml | ||||
| BOT53080 | Scientific Laboratory Supplies | PK10 | 39.6 EUR | |
Reagent Bottle Amber 100ml | ||||
| BOT64240 | Scientific Laboratory Supplies | PK10 | 98.4 EUR | |
Reagent Bottle Amber 250ml | ||||
| BOT64260 | Scientific Laboratory Supplies | PK10 | 82.8 EUR | |
Reagent Bottle Amber 500ml | ||||
| BOT64282 | Scientific Laboratory Supplies | PK10 | 126 EUR | |
Nitrate Reagent A - 10mL | ||||
| MIC6720 | Scientific Laboratory Supplies | EACH | 26.33 EUR | |
Nitrate Reagent B- 10mL | ||||
| MIC6722 | Scientific Laboratory Supplies | EACH | 26.33 EUR | |
Dissociation Reagent, 25ML | ||||
| X017-25ML | Arbor Assays | 25ML | 242 EUR | |
Allele-In-One Human Blood Direct Lysis Buffer | ||||
| ABP-PP-BD01100 | Allele Biotech | 100 RXNS | Ask for price | |
Allele-In-One Human Blood Direct Lysis Buffer | ||||
| ABP-PP-BD01500 | Allele Biotech | 500 RXNS | Ask for price | |
Reagent Bottles Amber 500ml | ||||
| BOT53080A | Scientific Laboratory Supplies | PK10 | 357.6 EUR | |
Reagent Reservoir 25ml NS | ||||
| F267660 | Scientific Laboratory Supplies | PK100 | 159.6 EUR | |
Indole Kovacs Reagent - 10mL | ||||
| MIC6734 | Scientific Laboratory Supplies | EACH | 26.33 EUR | |
Mouse IgG Blocking Reagent | ||||
| 30R-AH071 | Fitzgerald | 20 mg | 309.6 EUR | |
Bottle Reagent Amber Wn 250ml | ||||
| BOT64660 | Scientific Laboratory Supplies | PK10 | 114 EUR | |
MIDASplus HT-96 Single Reagent 100mL | ||||
| M-MDSR-107 | MiTeGen | 100 ml ml | 183 EUR | |
Creatinine Color Reagent, 28ML | ||||
| C004-28ML | Arbor Assays | 28ML | 254 EUR | |
cAMP Biotrak(TM) EIA; (Non-Acetylation Protocol; Lysis Reagents not Included); Cytiva; RPN2251 | ||||
| GERPN2251 | Scientific Laboratory Supplies | EACH | 685.2 EUR | |
Reagent Bottle Wide Clear 100ml | ||||
| BOT64440 | Scientific Laboratory Supplies | PK10 | 62.4 EUR | |
Reagent Bottle Wide Clear 250ml | ||||
| BOT64460 | Scientific Laboratory Supplies | PK10 | 81.6 EUR | |
EP Reagent Biuret Reagent | ||||
| 1011601 | Scientific Laboratory Supplies | 1L | 52.8 EUR | |
Simax Reagent Bottle Amber 1000ml | ||||
| BOT53100A | Scientific Laboratory Supplies | PK10 | 483.6 EUR | |
Pyrex Reagent Bottle 500ml Square | ||||
| 1520/08D | Scientific Laboratory Supplies | PK10 | 133.2 EUR | |
EP Reagent Iodoplatinate Reagent | ||||
| 1046300 | Scientific Laboratory Supplies | 200ML | 446.4 EUR | |
500ml Reagent Bottle Amber Narrow Mouth | ||||
| BOT55260 | Scientific Laboratory Supplies | PK2 | 35.04 EUR | |
Mouse-to-Mouse Blocking Reagent | ||||
| MTM008 | ScyTek Laboratories | 8 ml | 96 EUR | |
Mouse-to-Mouse Blocking Reagent | ||||
| MTM015 | ScyTek Laboratories | 15 ml | 105.6 EUR | |
Mouse-to-Mouse Blocking Reagent | ||||
| MTM125 | ScyTek Laboratories | 125 ml | 434.4 EUR | |
Mouse-to-Mouse Blocking Reagent | ||||
| MTM500 | ScyTek Laboratories | 500 ml | 991.2 EUR | |
EP Reagent Molybdovanadic Reagent | ||||
| 1056700 | Scientific Laboratory Supplies | 100ML | 52.8 EUR | |
Hemoglobin Detection Reagent, 20ML | ||||
| C038-20ML | Arbor Assays | 20ML | 242 EUR | |
The BCS Screen HT-96 Single Reagent 100mL | ||||
| M-MDSR-105 | MiTeGen | 100 ml ml | 155 EUR | |
Voges-Proskauer I Reagent 10mL | ||||
| MIC6726 | Scientific Laboratory Supplies | EACH | 26.33 EUR | |
Voges-Proskauer II Reagent 10mL | ||||
| MIC6728 | Scientific Laboratory Supplies | EACH | 26.33 EUR | |
EP Reagent Methoxyphenylacetic Reagent | ||||
| 1053601 | Scientific Laboratory Supplies | 100ML | 435.6 EUR | |
DetectX® Creatinine Reagent, 20ML | ||||
| C004-20ML | Arbor Assays | 20ML | 254 EUR | |
The BCS Screen 10 mL kit Single Reagent 100mL | ||||
| M-MDSR-104 | MiTeGen | 100 ml ml | 155 EUR | |
EP Reagent Sulfomolybdic Reagent R3 | ||||
| 1086500 | Scientific Laboratory Supplies | 1L | 354 EUR | |
Corning 3D Clear Tissue Clearing Reagent - 100mL | ||||
| 5733 | Scientific Laboratory Supplies | EACH | 1309.2 EUR | |
EP Reagent Phosphomolybdotungstic Reagent | ||||
| 1065000 | Scientific Laboratory Supplies | 100ML | 201.6 EUR | |
DeStreak reagent 1mL | ||||
| 17600318 | Scientific Laboratory Supplies | EACH | 82.8 EUR | |
The BCS Eco Screen HT-96 Single Reagent 100mL | ||||
| M-MDSR-105-ECO | MiTeGen | 100 ml ml | 155 EUR | |
Hach Total Chlorine Reagent For 25ml | ||||
| 1406499 | Scientific Laboratory Supplies | PK100 | 55.2 EUR | |
FRAP Reagent A, 1.4ML | ||||
| C148-1.4ML | Arbor Assays | 1.4ML | 150 EUR | |
FRAP Reagent B, 1.4ML | ||||
| C149-1.4ML | Arbor Assays | 1.4ML | 150 EUR | |
EP Reagent Bromothymol Blue Sol. R3 500mL | ||||
| 1012903 | Scientific Laboratory Supplies | PK2 | 345.6 EUR | |
Comparison of Single-Tube Systems
The Viagen DirectPCR system is a single-tube DNA extraction system that is PCR-ready. This system is compared to other single-tube systems on the market, and it is found to be more efficient and easier to use. The Viagen DirectPCR system can be used with a variety of sample types, and it provides high yields of DNA. This system is also relatively inexpensive, making it a good option for those who are looking for an affordable DNA extraction system.