If you are working with DNA, you may be looking for an easier and more efficient way to extract it. With increased accessibility to computer-controlled instruments and reagents, as well as development of specialized protocols, there have been many advances in the field of biotechnology in recent years. One such advancement is the Viagen DirectPCR DNA Extraction System, which allows you to quickly prepare high quality DNA from various samples types including mouse tails, ear pieces, yolk sacs, and culture cells.
Why Use Viagen DirectPCR DNA Extraction System?
There are many reasons to use the Viagen DirectPCR DNA Extraction System. This system is PCR ready, which means that it is able to be used with a polymerase chain reaction machine. This system is also able to extract DNA from a variety of different sources, including blood, tissue, and even plant material. In addition, this system is very easy to use and can be completed in just a few minutes. Who Needs Viagen DirectPCR DNA Extraction System?. Viagen DirectPCR DNA Extraction System is recommended for anyone who needs to extract DNA samples from a sample. Whether you are a researcher, scientist, forensic expert, or even simply someone looking to learn more about genetics and genealogy, this system is useful for everyone.
What Are the Features of Viagen DirectPCR DNA Extraction System?. Here are some of the key features of this system:
• PCR ready – includes all necessary components to use with polymerase chain reaction machines
• Can extract DNA from blood and tissue samples as well as plant material such as seeds or leaves
• Extracts less than one microgram of DNA
How to Use Viagen DirectPCR DNA Extraction System
If you’re looking for a quick and easy way to extract DNA from cells, the Viagen DirectPCR system is perfect for you. This PCR-ready system can be used with any cell type and doesn’t require any special equipment or knowledge – all you need is a few minutes and some basic lab supplies. Here’s how it works:
1) First, add the appropriate amount of lysis buffer to your sample. Lysis buffer breaks open cells and releases their contents, including DNA.
2) Next, incubate the sample at 95°C for 10 minutes. This step denatures proteins that could interfere with DNA extraction.
3) Then, add the DirectPCR enzyme mix to the sample and incubate for 5 minutes at room temperature. The enzymes in the mix will bind to and break down DNA.
4) Finally, add your favorite PCR master mix and run your PCR reaction as usual. You’ll now have PCR-ready DNA that’s ready to use in any experiment! DirectPCR® is an enzyme mix that allows you to directly purify DNA from cells, tissues, or plant material in just minutes. The kit also includes a special buffer that eliminates the need for extraction and precipitation steps during PCR setup. DirectPCR uses a proprietary blend of enzymes to break down DNA into smaller fragments that are compatible with PCR assays. Use this handy kit to generate your own genomic DNA for applications like genotyping and sequencing!
Note: This product is supplied as a dry powder. It will be necessary to add water before use.
Where to Buy the Viagen DirectPCR DNA Extraction System?
If you’re looking for a PCR-ready DNA extraction system, the Viagen DirectPCR system is a great option. This system is designed for use with whole blood, so it’s perfect for extracting DNA from samples that contain blood cells. The Viagen DirectPCR system is available from several different vendors, so you can choose the one that best suits your needs. For example:
Viagen DirectPCR DNA Extraction System (Cat # V5202) is available from B&J Biotech for $1,400.00. This kit includes a 96-well Thermal Cycler with a software package that allows you to monitor PCR reactions remotely via the web. In addition, it includes all the supplies needed to prepare and purify genomic DNA from whole blood samples. The Viagen DirectPCR system has been tested for use with human blood samples, but you will need to purchase the BioRobot eXtractor machine separately if you want to process samples of other species. This system comes with everything you need to start extracting DNA from whole blood samples in order to do PCR testing.
DIRECTPCR LYSIS REAGENT (TAIL) | ||||
101-T | Viagen | 50 ml | 141 EUR | |
DIRECTPCR LYSIS REAGENT (TAIL) | ||||
102-T | Viagen | 100ml | 207 EUR | |
DIRECTPCR LYSIS REAGENT (CELL) | ||||
301-C | Viagen | 50 ml | 141 EUR | |
DIRECTPCR LYSIS REAGENT (CELL) | ||||
302-C | Viagen | 100 ml | 207 EUR | |
DIRECTPCR LYSIS REAGENT (EAR) | ||||
401-E | Viagen | 25 ml | 141 EUR | |
DIRECTPCR LYSIS REAGENT (EAR) | ||||
402-E | Viagen | 50 ml | 207 EUR | |
DetectX® Creatinine Reagent, 50ML | ||||
C004-50ML | Arbor Assays | 50ML | 359 EUR | |
DIRECTPCR LYSIS RGNT(YOLK SAC) | ||||
201-Y | Viagen | 50 ml | 141 EUR | |
DIRECTPCR LYSIS YOLK SAC 100ML | ||||
202-Y | Viagen | 100 ml | 207 EUR | |
Assay Buffer, 50ML | ||||
X100-50ML | Arbor Assays | 50ML | 109 EUR | |
Assay Buffer, 50ML | ||||
X117-50ML | Arbor Assays | 50ML | 143 EUR | |
Extraction Solution, 50ML | ||||
X123-50ML | Arbor Assays | 50ML | 123 EUR | |
Purified Water, 50ML | ||||
X137-50ML | Arbor Assays | 50ML | 122 EUR | |
Hemoglobin Sample Diluent, 50ML | ||||
X052-50ML | Arbor Assays | 50ML | 153 EUR | |
RBC Lysis Solution 10X | ||||
abx290033-50ml | Abbexa | 50 ml | 230 EUR | |
RBC Lysis Solution 10X | ||||
RBC10X-50ML | ImmunoStep | 50 ML | 108.5 EUR | |
mRNAExpress Tail PCR mix (5uM) | ||||
MR-TAIL-PR | SBI | 100 ul | 164 EUR | |
TARGATT? H11 Mouse Tail Lysis PCR Kit | ||||
AST-2005 | Applied StemCell | 100 reactions | 130.6 EUR | |
TARGATT? Rosa26 Mouse Tail Lysis PCR Kit | ||||
AST-2006 | Applied StemCell | 100 reactions | 130.6 EUR | |
Folin & Ciocalteu’s phenol reagent | ||||
abx082175-50ml | Abbexa | 50 ml | 189 EUR | |
Allele-In-One Mouse Tail Direct Lysis Buffer | ||||
ABP-PP-MT01100 | Allele Biotech | 100 RXNS | Ask for price | |
Allele-In-One Mouse Tail Direct Lysis Buffer | ||||
ABP-PP-MT01500 | Allele Biotech | 500 RXNS | Ask for price | |
SingleStep Poly-HRP Anti-Mouse IgG (H+L) Secondary Antibody, HRP-50ml | ||||
Q2AB1-50ml | EnQuireBio | 50ml | 403 EUR | |
Xpro IP Cell Lysis Reagent,SDS Free | ||||
X3100-010 | GenDepot | 100ml | 174 EUR | |
Xpro IP Cell Lysis Reagent,SDS Free | ||||
X3100-050 | GenDepot | 500ml | 317 EUR | |
50ML , CONICAL BOTTOMED TUBE WITH CAP, UNASSEMBLED | ||||
SCT-50ML-500 | CORNING | 50/pk | 87 EUR | |
SingleStep Poly-HRP Anti-Rabbit IgG (H+L) Secondary Antibody, HRP-50ml | ||||
Q2AB2-50ml | EnQuireBio | 50ml | 403 EUR | |
REAGENT RESERV, 50ML,WHITE,PS,S,BK,5/200 | ||||
4870 | CORNING | 5/pk | 190 EUR | |
REAGENT RESERV,50ML,WHITE,PS,S,IND,1/100 | ||||
4871 | CORNING | 1/pk | 218 EUR | |
50ML , CONICAL BOTTOMED TUBE WITH CAP, ASSEMBLED, PRE-STERILIZED | ||||
SCT-50ML-25-S | CORNING | 25/pk | 98 EUR | |
50ML , CONICAL BOTTOMED TUBE WITH CAP, RACKED, ASSEMBLED, PRE-STERILIZED | ||||
SCT-50ML-R-S | CORNING | 250/pk | 106 EUR | |
Lysis Buffer | ||||
abx098984-LysisBuffer120ml | Abbexa | Lysis Buffer 1 (20 ml) | 154 EUR | |
Lysis Buffer | ||||
abx098984-LysisBuffer3100ml | Abbexa | Lysis Buffer 3 (100ml) | 230 EUR | |
Lysis Buffer | ||||
abx098984-LysisBuffer420ml | Abbexa | Lysis Buffer 4 (20 ml) | 154 EUR | |
Mouse IgG Blocking Reagent | ||||
30R-AH071 | Fitzgerald | 20 mg | 258 EUR | |
Burettes Brush, 50ml | ||||
BR004-50 | Bio Basic | 1 UNIT | 51.74 EUR | |
CRYOPRESERVATION BAG, 50ML | ||||
91-200-88 | CORNING | 1/pk | 81 EUR | |
TubeSpin Bioteactor (50mL) | ||||
TS50 | Next Advance | 180case | 65 EUR | |
50ML 2-Mercaptoethanol | ||||
NAT1070 | National Diagnostics | 50ML | 94 EUR | |
Background Buster, 50ml | ||||
NB306-50 | Innovex | 50 ml | 396 EUR | |
ExoQuick TC 50ml | ||||
EXOTC50A-1 | SBI | 50 ml | 1222 EUR | |
Mouse-to-Mouse Blocking Reagent | ||||
MTM008 | ScyTek Laboratories | 8 ml | 80 EUR | |
Mouse-to-Mouse Blocking Reagent | ||||
MTM015 | ScyTek Laboratories | 15 ml | 88 EUR | |
Mouse-to-Mouse Blocking Reagent | ||||
MTM125 | ScyTek Laboratories | 125 ml | 362 EUR | |
Mouse-to-Mouse Blocking Reagent | ||||
MTM500 | ScyTek Laboratories | 500 ml | 826 EUR | |
Cell Lysis Buffer | ||||
AR0103 | BosterBio | 100mL (The kit contains sufficient lysis buffer for 200 cell pellet fractions containing 5x 106 cells each) | 121 EUR | |
RIPA Lysis Buffer | ||||
AR0105 | BosterBio | 50 mL | 75 EUR | |
IP Lysis Buffer | ||||
AR0107 | BosterBio | 100mL | 101 EUR | |
CometAssay Lysis Solution | ||||
4250-010-01 | Biotechne | 100 mL | 157.8 EUR | |
ExCellenCT Lysis Kit | ||||
G915 | ABM | 25 Preps | 92 EUR | |
Cell Lysis Buffer | ||||
1067-100 | Biovision | 164 EUR | ||
Cell Lysis Buffer | ||||
1067-400 | Biovision | 294 EUR | ||
Bradford reagent | ||||
BDE641 | Bio Basic | 100ml | 61.01 EUR | |
Beaucage reagent | ||||
HY-100951 | MedChemExpress | 10mM/1mL | 126 EUR | |
BOP reagent | ||||
5-02141 | CHI Scientific | 25g | Ask for price | |
BOP reagent | ||||
5-02142 | CHI Scientific | 100g | Ask for price | |
Bluing Reagent | ||||
BRT030 | ScyTek Laboratories | 30 ml | 60 EUR | |
Bluing Reagent | ||||
BRT125 | ScyTek Laboratories | 125 ml | 63 EUR | |
Bluing Reagent | ||||
BRT3800 | ScyTek Laboratories | 1 Gal. | 184 EUR | |
Bluing Reagent | ||||
BRT500 | ScyTek Laboratories | 500 ml | 76 EUR | |
Bluing Reagent | ||||
BRT999 | ScyTek Laboratories | 1000 ml | 88 EUR | |
BOP reagent | ||||
A7015-100000 | ApexBio | 100 g | 200 EUR | |
BOP reagent | ||||
A7015-25000 | ApexBio | 25 g | 113 EUR | |
Traut's Reagent | ||||
2330-1000 | Biovision | 349 EUR | ||
Traut's Reagent | ||||
2330-500 | Biovision | 207 EUR | ||
MTS Reagent | ||||
2808-1000 | Biovision | 990 EUR | ||
MTS Reagent | ||||
2808-250 | Biovision | 365 EUR | ||
MTT Reagent | ||||
2809-1G | Biovision | 180 EUR | ||
MTT Reagent | ||||
2809-5G | Biovision | 544 EUR | ||
Chymase reagent | ||||
30C-CP1129 | Fitzgerald | 5 units | 2185 EUR | |
COOLRACK 50ML, HOLDS 4 X 50ML CONICAL CENTRIFUGE TUBE | ||||
432062 | CORNING | 1/pk | 308 EUR | |
Mouse Collagen I (Tail Tendon) Protein | ||||
abx670020-05mg | Abbexa | 0.5 mg | 356 EUR | |
Graduated Plastic Beakers, 50ml | ||||
BK044-2 | Bio Basic | 1 UNIT | 52.18 EUR | |
CORNING, SYRINGE TIPS, 50ML | ||||
6627 | CORNING | 1/pk | 58 EUR | |
50ML Protein Precipitation Kit | ||||
NAT1254 | National Diagnostics | 50ML | 225 EUR | |
CD2 Tail-binding Antibody | ||||
34187-100ul | SAB | 100ul | 252 EUR | |
CD2 Tail-binding Antibody | ||||
34187-50ul | SAB | 50ul | 187 EUR | |
CD2 Tail-binding Antibody | ||||
45412-100ul | SAB | 100ul | 252 EUR | |
CD2 Tail-binding Antibody | ||||
45412-50ul | SAB | 50ul | 187 EUR | |
CD2 Tail-binding Antibody | ||||
DF8644 | Affbiotech | 200ul | 304 EUR | |
CD2 Tail- binding Antibody | ||||
ABD13035 | Lifescience Market | 100 ug | 438 EUR | |
CD2 Tail- binding Antibody | ||||
ABD8644 | Lifescience Market | 100 ug | 438 EUR | |
CD2 Tail-binding antibody | ||||
70R-32334 | Fitzgerald | 100 ug | 327 EUR | |
RIPA Lysis Buffer (Strong) | ||||
HY-K1001 | MedChemExpress | 100 mL | 147 EUR | |
Green Lysis Kit 50 | ||||
GREENE1 | Next Advance | 50pack | 156 EUR | |
Green Lysis Kit 250 | ||||
GREENE5 | Next Advance | 250pack | 468 EUR | |
Orange Lysis Kit 50 | ||||
ORANGEE1-RNA | Next Advance | 50pack | 165 EUR | |
Orange Lysis Kit 250 | ||||
ORANGEE5-RNA | Next Advance | 250pack | 537 EUR | |
Pink Lysis Kit 50 | ||||
PINKE1 | Next Advance | 50pack | 154 EUR | |
Pink Lysis Kit 250 | ||||
PINKE5 | Next Advance | 250pack | 457 EUR | |
Navy Lysis Kit 50 | ||||
NAVYE1 | Next Advance | 50pack | 175 EUR | |
Navy Lysis Kit 250 | ||||
NAVYE5 | Next Advance | 250pack | 547 EUR | |
RIPA Lysis Buffer (Strong) | ||||
abx090624-100ml | Abbexa | 100 ml | 217 EUR | |
RIPA Lysis Buffer (Medium) | ||||
abx090625-100ml | Abbexa | 100 ml | 217 EUR | |
RIPA Lysis Buffer (Weak) | ||||
abx090626-100ml | Abbexa | 100 ml | 217 EUR | |
5X Passive Lysis Buffer | ||||
99912 | Biotium | 30ML | 77 EUR | |
Exosomal Protein Lysis Buffer | ||||
P200P,-part-of-P200 | 101Bio | - | Ask for price | |
CHAPS Cell Lysis Buffer | ||||
C2000-100 | GenDepot | 100ml | 138 EUR | |
CHAPS Cell Lysis Buffer | ||||
C2000-500 | GenDepot | 500ml | 334 EUR | |
Red Lysis Kit 50 | ||||
REDE1 | Next Advance | 50pack | 168 EUR |
Comparison of Single-Tube Systems
The Viagen DirectPCR system is a single-tube DNA extraction system that is PCR-ready. This system is compared to other single-tube systems on the market, and it is found to be more efficient and easier to use. The Viagen DirectPCR system can be used with a variety of sample types, and it provides high yields of DNA. This system is also relatively inexpensive, making it a good option for those who are looking for an affordable DNA extraction system.